By Ute Resch-Genger, Markus Grabolle, Roland Nitschke, Thomas Nann (auth.), Alexander P. Demchenko (eds.)
From the reviews:
“This e-book is a complete evaluate of the differing kinds of nanoscale probes which have been and are presently being constructed for sensing of analytes. The well timed reference part permits the reader to find key references to the first literature for growth of the fabric coated. … a worthwhile source that covers the unreal, photo-physical, and theoretical points of this assorted box of research.” (Jeffrey T. Petty, magazine of the yank Chemical Society, Vol. 133, 2011)
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Additional info for Advanced Fluorescence Reporters in Chemistry and Biology II: Molecular Constructions, Polymers and Nanoparticles
This approach requires sufficiently different lifetimes of the chromophores. With a single exception, lifetime multiplexing, as well as a combined spectral and lifetime discrimination have only been realized with organic chromophores . This is most likely, related to the fact that the need for monoexponential decay kinetics was often assumed for this application. Meanwhile, successful lifetime multiplexing has been also reported both for a mixture of a QD and an organic dye and for a mixture of two different QDs  despite the multiexponential decay kinetics of the QDs.
Similar drawbacks arise for all types of fluorophoredoped nanoparticles. Only recently, methods have been developed to optimize the 1:1 stochiometry of QD-biomolecule conjugates . 2 23 Extra- and Intracellular Targeting of Biomolecules The location and dynamics of biomolecules like proteins play an important role in cell signal transduction. , for cancer research and target quantification. , on the cell surface or inside cells, and needs to be met by any fluorescent label suitable for this purpose.
He GS, Yong K, Zheng Q, Sahoo Y, Baev A, Ryasnyanskiy AI, Prasad PN (2007) Multiphoton excitation properties of CdSe quantum dots solutions and optical limiting behavior in infrared range. Opt Exp 15:12818–12833 63. Clapp AR et al (2007) Two-Photon excitation of quantum-dot-based fluorescence resonance energy transfer and its applications. Adv Mater 19:1921–1926 64. Jaiswal JK, Simon SM (2004) Potentials and pitfalls of fluorescent quantum dots for biological imaging. Trends Cell Biol 14:497–504 65.
Advanced Fluorescence Reporters in Chemistry and Biology II: Molecular Constructions, Polymers and Nanoparticles by Ute Resch-Genger, Markus Grabolle, Roland Nitschke, Thomas Nann (auth.), Alexander P. Demchenko (eds.)