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By Jean Effront

ISBN-10: 1117093131

ISBN-13: 9781117093130

Initially released in 1917. This quantity from the Cornell collage Library's print collections used to be scanned on an APT BookScan and switched over to JPG 2000 layout by means of Kirtas applied sciences. All titles scanned conceal to hide and pages may possibly contain marks notations and different marginalia found in the unique quantity.

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3% potassium oxalate is added and the precipitate of calcium oxalate is removed by centrifuging and filtering. The filtered liquid is then diluted with two or three volumes of water, filtered again to eliminate the last traces of insoluble matter, and then acidified with acetic acid. A precipitate is formed, which contains thrombiA. This is col- PREPARATION OF THROMBIN AND FIBRINOGEN 35 lected and purified by dissolving in water made slightly alkaline, and reprecipitating with acetic acid. To obtain a sufficiently pure product, it is necessary to repeat this treatment a number of times.

Duclaux has adopted the term of plasmase, but it is also designated by the names of 33 34 BIOCHEMICAL CATALYSTS fibrinase, thrombin, or even of thrombase. We shall adopt the term thrombin, since, by reason of the different theories of coagulation given out, it leads to less confusion than any other. Thrombin was discovered by Buchanan in 1835. This enzyme, or rather one of the constituent parts of this enzyme, is found in abundance in the white corpuscles of the blood. I t is also found in the red corpuscles, though in much smaller proportion, and in many tissues.

I t is true that heating does not release all the fibrinogen contained in the plasma. The fact, then, that the weight of fibrin collected is slightly less than that of fibrinogen obtained by coagulation leads only to the conclusion that this weight of • fibrin is clearly smaller than the actual weight of fibrinogen contained in the plasma. But this reasoning is not an actual proof. On the other hand, Bordet and Gengou have shown, while studying the comparative action of an emulsion of BaSO4 on serum exuded from a clot and on serum obtained by defibrination of blood in process of coagulation, that, in the first case the mixture remains turbid indefinitely, while in the second the precipitate is rapidly deposited, due to an actual clarifying effected by the fibrin remaining in solution in the blood.

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biochemical catalysts in life and industry by Jean Effront


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