By Bernard Pullman (auth.), Bernard Pullman (eds.)
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Extra info for Catalysis in Chemistry and Biochemistry Theory and Experiment: Proceedings of the Twelfth Jerusalem Symposium on Quantum Chemistry and Biochemistry held in Jerusalem, Israel, April 2–4, 1979
1970, J. Colloid. Interface Sci 33, pp. 124-132. : 1969, J. Colloid. Interface Sci. 29, pp. 36-41. : 1959, J. Colloid. Interface Sci. 14, pp. 161-165. : 1973, Anal. Biochem. 51, pp. 127136. THEORETICAL STUDIES ON MODELS OF THE ACTIVE SITE OF CARBONIC ANHYDRASE D. DemouLin and A. S. 13, rue Pierre et Marie Curie - 75005 Paris. ABSTRACT TheoreticaL ab initio pseudopotentiaL SCF computations performed on a model of the Glu 106 - Thr 199 ~ water H-bonded chain, free and bound to the zinc cation at the active site of carbonic anhydrase show that whereas the GLu - Thr - water form of the unbound chain is stabLe, the same chain, when bound to zinc, is more stabLe in the GLu - Thr - OH- form, and the proton-reLay process to that form occurs with no barrier.
KIRBY ~OR ~OH (6) o~ We have tested this point by a comparison of another pair of compounds, (8)and (9). The hydrolysis of(8) shows efficient intramolecular general acid catalysis, by the mechanism shown (Kirby and Osborne, unpublished). /OMe I ~e I (8) ° (9) But the related enol acetal (9), which has an identical leaving group, and differs from (8) only in that the COOH is no longer conjugated with the leaving group (and in the small change in their geometrical relationship) shows only very weak intramolecular catalysis.
Behavior and use of micromicellar solutions at subzero temperatures At room temperature, most micromicellar solutions are homogeneous and optically transparent. They are in fact "colorless" down to 350-250 nm according to the type of surfactant used. These solutions must retain these optical properties in a given range of subzero temperatures to be used as reaction medium for cryoenzymologic studies. The temperature at which transparency is changing can be easily determined and solubility diagrams recorded.
Catalysis in Chemistry and Biochemistry Theory and Experiment: Proceedings of the Twelfth Jerusalem Symposium on Quantum Chemistry and Biochemistry held in Jerusalem, Israel, April 2–4, 1979 by Bernard Pullman (auth.), Bernard Pullman (eds.)